Varieties from different оriental tobacco ecotypes are a great sensitivity to the conditions of the environment. In recent years, some varieties of tobacco have been grown in areas other than those for which they were selected. This necessitates to study their plasticity, morphology and quality in different untypical conditions. During the period 2021-2022, biometric, quality and some basic chemical indicators were investigated in the dry tobacco of the four varieties of Krumovgrad 90, Elenski 817, Dupnitsa 160 and Dzebel 576 belonging to four different ecotypes (Ecotype Krumovgrad, Ecotype Eastern Balkans, Ecotype Dupnitsa and Ecotype Dzebel Basma). The experiments were conducted in an еxperimental field (EF)-Markovo. The values of chemical indicators were determined in an Accredited Laboratory Complex (s) to TTPI. The results show that climatic conditions are a determining factor for plant development and for the quality of the raw material formed, with better biometric indicators reported in 2022. The investigated varieties have a relatively high percentage of the I-st class. In the conditions of EF - Markovo, the highest nicotine values were reported in the variety Elenski 817. Nicotine values in Dupnitsa 160 and Krumovgrad 90 varieties are lower in tobacco from the 2022 harvest. Climate conditions have a great impact on morphological and chemical indicators in the studied varieties.